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. 2011 Nov 28;195(5):827–838. doi: 10.1083/jcb.201105144

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© 2011 Pocha et al.

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Figure 4. — MyoV stabilization does not require an intact Crb complex. (A) Section through an adult retina harboring crb^8F105 large clones stained for MyoV and F-actin. Arrows highlight MyoV enrichment at the base of the rhabdomere, as seen in WT photoreceptors. (B) Western blots from whole-cell lysates of WT retinas and retinas harboring large clones of crb^11A22 or crb^8F105. Tubulin (Tub) is used as a loading control. The markers shown are for molecular masses in kilodaltons. (C) An S2R+ cell expressing MyoV-GFP and plated on concanavalin A shows a punctate distribution throughout the cell. (D and E) S2R+ cells coexpressing MyoV-GFP and Crb and plated on concanavalin A show recruitment of MyoV-GFP to the plasma membrane, where it colocalizes with Crb. Bars, 5 µm.