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. 2011 Nov 28;195(5):827–838. doi: 10.1083/jcb.201105144

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© 2011 Pocha et al.

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Figure 7. — Crb mutant PRCs display slower Rh1 transport kinetics. (A) Schematic of the Rh1 pulse-chase experiment. Red dots symbolize immature Rh1 containing all-trans-retinal. Blue dots symbolize mature Rh1 containing 11-cis-retinal (for a detailed description, see Results and Materials and methods). (B and C) Rh1 pulse-chase experiment. Sections through adult retinas containing crb^11A22 clones from flies that were fed on carotenoid-free medium from egg to adult, given all-trans-retinal for 2 d in the dark, and then pulsed with CFP-filtered light for 10 min and returned to the dark for the times indicated. Sections were stained for Rh1 and F-actin. Borders between WT and crb^11A22 (crb) tissue are outlined in white. The structure of the eye is not as well preserved as those in Fig. 5 A because the flies are fixed whole to avoid exposure to light before fixation. Bars, 5 µm.