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. Author manuscript; available in PMC: 2012 Jan 13.

Published in final edited form as: Exp Cell Res. 2008 Dec 16;315(5):820–835. doi: 10.1016/j.yexcr.2008.12.003

(A) Kinetics of mant-GDP release from 100 pM His-Rab5 in the absence ( ) and presence of 0.01 μM ( ), 0.1 μM ( ), 1 μM (←) Rin1: wild type proteins or following the addition of 6 mM EDTA ( ) for the indicated time at 30°C. The data are presented as means ± SD of three independent experiments, n=3 *P < 0.001. (B) Release of mant-GDP from 100 pM His-Rab5 alone ( ) or in the presence of 0.1 μM Rin1 constructs for the indicated time at 30°C as described in Material and Methods. The data are presented as means ± SD of three independent experiments, n=3 *P < 0.001. Each independent experiment was carried out in duplicate. Relative Units (R.U.) indicate the amount of fluorescence measured at 440 nm (I440 nm).

Inline graphic — (A) Kinetics of mant-GDP release from 100 pM His-Rab5 in the absence ( ) and presence of 0.01 μM ( ), 0.1 μM ( ), 1 μM (←) Rin1: wild type proteins or following the addition of 6 mM EDTA ( ) for the indicated time at 30°C. The data are presented as means ± SD of three independent experiments, n=3 *P < 0.001. (B) Release of mant-GDP from 100 pM His-Rab5 alone ( ) or in the presence of 0.1 μM Rin1 constructs for the indicated time at 30°C as described in Material and Methods. The data are presented as means ± SD of three independent experiments, n=3 *P < 0.001. Each independent experiment was carried out in duplicate. Relative Units (R.U.) indicate the amount of fluorescence measured at 440 nm (I440 nm).