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. Author manuscript; available in PMC: 2012 Jan 13.
Published in final edited form as: Apoptosis. 2011 Nov;16(11):1150–1164. doi: 10.1007/s10495-011-0645-6

Fig. 5.

Fig. 5

Heterogeneous response of neurospheres to rhTRAIL treatment. A Cell viability analysis of TRAIL-induced cell death in two passages (p3, p7) of NSC189 and NSC326 after treatment with 300 ng/ml rhTRAIL for 24 hr. Data are mean +/− SEM (n = 6). B–C Caspase-8 and Caspase-3/-7 activity measured in two passages of NSC326 and NSC189 after rhTRAIL treatment for 24 hrs in the presence or absence of caspase-8 inhibitor Z-IEDT and pan-caspase inhibitor z-VAD. Data are mean +/− SEM (n = 3). D Western blot analysis of the expression of DR5, FADD, caspase-8 (Casp-8) in the p3 and p7 passage of NSC326 and NSC189 with primary cultures (p) and clones (c12, c16, c13, c17) included as controls (the molecular weights are indicated to the right). Actin was used as the protein loading control.