Table 3.
Sequences and melting temperatures of primers designed on new sequences.
| Name | Sequence | Melting temp. (°C ) |
|---|---|---|
| Orange forward | 5’-GGCACGGGTTAAGTAGATTTGC-3’ | 60.3 |
| Orange reverse | 5’-TTATATGTTCGCGCTGGTATGATC-3’ | 57.1 |
| Blueberry forward | 5’-CGACCTTGGCGGAAAACA-3’ | 56.0 |
| Blueberry reverse | 5’-AAGTGAGTTCCCTCCACTTTCG-3’ | 60.0 |
| Pineapple forward | 5’-GGAGGAGCCCGAAAAACG-3’ | 58.2 |
| Pineapple reverse | 5’-TTTCCGCCTTCTCAAGCAGTT-3’ | 57.9 |
| Strawberry forward | 5’-CGAAAGGGCAAGGAAAAATG-3’ | 55.3 |
| Strawberry reverse | 5’-GCTCCTCCCGAGCTCATCT-3’ | 61.0 |