Figure 9.
.Effect of SLPE feeding on immunohistochemical detection of rotavirus in intestinal tissue from rotavirus-infected piglets. Piglets were inoculated with rotavirus (1 × 105 FFU) in the presence of MEM or SLPE (2 µmol) and fed MEM or SLPE (2 µmol) every 12 h, euthanized 28–96 h post-inoculation, and intestinal tissue harvested and processed for immunohistochemical detection of rotavirus antigen as described in the Experimental Section. Typical photomicrographs of immunohistochemically stained sections of small intestinal segments from control and SLPE-fed, virus-infected piglets are shown below. (A) rotavirus-inoculated pig fed MEM (40 h); (B) rotavirus-inoculated pig fed SLPE (40 h).