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Phosphorylation of dThd, N5 (3), N5-Br (12a/b), N5-I (4a/b), and N5-I4 (5) by recombinant TK1. Assay products were separated by PEI–cellulose TLC.
a: Monophosphates of N5 (3) N5-I (4a/b), and N5-Br (12a/b).
b: Unidentified phosphorylation products with relative phosphorylation rates of 14 ± 0.7 % (N5-I lane) and 25 ±1.1 % (N5-Br lane)
