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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Oral Microbiol. 2011 Oct 4;27(1):34–44. doi: 10.1111/j.2041-1014.2011.00627.x

Figure 2.

Figure 2

Proteomic analysis by SDS- PAGE, and Western blot assay of extracts from cells of L. buccalis 14201 grown on various sugars. A. SDS-PAGE and visualization of proteins by staining with Coomassie blue R-250. Approx. 30μg of protein was applied per lane. Note the expression (white arrowheads) of a 50 kDa protein induced by growth of the organism on the four isomers of sucrose. B. Western blot of a duplicate gel showing the immuno-cross reactivity of the induced 50 kDa protein with polyclonal antibody raised against the NAD+ and Mn2+ -dependent phospho-α-glucosidase (MalH) from F. mortiferum.