Table 1.

Methodological differences in slice preparation and electrophysiological recording conditions.

Valenzuela lab methods	Lee et al. (2010) methods
P22–28 Sprague-Dawley rat and/or P28–P30 C57/B6 mouse	P28 or >8 weeks old C57/B6 mouse
Sucrose cutting solution (in mM): 220 sucrose, 2 KCl, 1.25 NaH2PO4, 26 NaHCO3, 12 MgSO4, 10 glucose, 0.2 CaCl2, and 0.43 ketamine	Sucrose cutting solution (in mM): 250 sucrose, 2.5 KCl, 1.25 NaH2PO4, 26 NaHCO3, 4 MgCl2, 10 glucose, 0.1 CaCl2, 3 myo-inositol, 2 sodium pyruvate, 0.5 ascorbic acid, and 1 kynurenic acid, pH 7.4
aCSF (in mM): 126 NaCl, 2 KCl, 1.25 NaH2PO4, 26 NaHCO3, 10 glucose, 1 MgSO4, 2 CaCl2, 0.4 ascorbic acid	aCSF (in mM): 126 NaCl, 2.5 KCl, 1 NaH2PO4, 24 NaHCO3, 10 glucose, 2 MgCl2. 2.5 CaCl2, pH 7.4
Internal solution (in mM): 135 KCl, 10 HEPES, 2 MgCl2, 0.5 EGTA, 5 Mg-ATP, 1 Na-GTP, and 1 QX314-(Br), pH 7.25 adjusted with KOH (280–290 mOsm)	Internal solution (in mM): 135 CsCl, 10 HEPES, 4 NaCl, 0.5 CaCl2, 5 EGTA, 2 Mg-ATP, 0.5 Na2-GTP, 10 QX-314, pH adjusted to 7.2 with CsOH (278–285 mOsmol)
Incubation protocol: 40 min at 32–33°C then at least 20–30 min at room temp	Incubation protocol: room temperature for at least 1 h prior to recording
Holding potential: −70 mV	Holding potential: −70 mV
Pipette resistance: 3–5 MΩ	Pipette resistance: 10–12 MΩ