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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Clin Immunol. 2011 Aug 23;141(2):205–217. doi: 10.1016/j.clim.2011.08.004

Figure 1. Anti-FcεR1 activates basophils and mast cells in vitro.

Figure 1

(A) May-Grünwald staining of peritoneal mast cells after enrichment by density centrifugation. (B) Histamine release from mast cells in response to increasing concentrations of anti-FcεR1. (C) Representative dot plots of intracellular IL-4 staining of basophils after incubation with media or anti-FcεR1. Initial gating of lymphocytes and lower half of granulocyte region in FSC/SSC plot (left panel). Basophils identified as CD4-B220-IgE+ (middle panel). IL-4 staining of basophils after incubation with media (upper right panel) or anti-FcεR1 (lower right panel). (D) Percentages of basophils that stain positively for IL-4 after incubation with media or anti-FcεR1. (E) IL-4 release from whole blood after incubation with anti-FcεR1. Error bars denote SEM. Results from 4-6 animals per group are shown. Each experiment was independently performed twice. Statistical significance assessed per one-tailed paired T-test.