Table 2.
Receptor and ligand densities measured by ELISA (receptors on nanoparticles and ligands on flow chamber substrates) or flow cytometry (receptors on microparticles).
| Type | Molecule | Molecular Density (•m−2) | |||
|---|---|---|---|---|---|
| Low | Med | High | Max | ||
| Receptor (Nanoparticle) | 4-4-20 scFv | 6,900 ± 900 | 14,100 ± 700 | 19,800 ± 700 | |
| 4M2.4 scFv | 4,800 ± 400 | 16,400 ± 1000 | 20,700 ± 1300 | ||
| 4M5.3 scFv | 3,600 ± 300 | 14,800 ± 600 | 20,000 ± 700 | ||
| 4-4-20 scFv-mRFP | 6,700 ± 500 | 20,400 ± 900 | |||
| 4-4-20 Ab | 480 ± 30 | 960 ± 130 | 2,500 ± 60 | ||
| Biotin | 680 ± 50 | 1,100 ± 150 | 1,500 ± 350 | 5,800 ± 100 | |
| VIII scFv | 390 ± 80 | 1,900 ± 200 | 19,600 ± 1200 | ||
| VIII scFv-mRFP | 400 ± 80 | 980 ± 200 | |||
| Receptor (Microparticle) | 4-4-20 scFv | 1200 ± 10 | 4600 ± 40 | ||
| 4M2.4 scFv | 630 ± 10 | 4600 ± 90 | |||
| 4M5.3 scFv | 640 ± 10 | 4600 ± 80 | |||
| Biotin | 2100 ± 170 | 5,600 ± 140 | |||
| Ligand | Fluorescein | 47 ± 4 | 76 ± 7 | 120 ± 8 | 195 ± 3 |
| Neutravidin | 29 ± 4 | 41 ± 7 | 154 ± 8 | 195 ± 3 | |
| VCAM-1 | 142 ± 9 | 457 ± 6 | |||