Recombinant protein analysis by SDS-PAGE and Western blotting. Lsa20 (A) and rLIC11030 (B) protein expression from NaCl-induced E. coli BL21-SI and IPTG-induced E. coli BL21(DE3) Star pLysS cultures, respectively. Lanes: 1 and 6, molecular mass protein marker; 2, noninduced total bacterial extract; 3, total bacterial cell lysates after induction; 4 and 5, soluble and insoluble (pellet) fractions of the induced culture, respectively; 7, purified recombinant proteins in Coomassie blue-stained gels; 8, Western blotting of the recombinant proteins probed with anti-His tag antibodies.