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. 2011 Nov;79(11):4657–4667. doi: 10.1128/IAI.05583-11

Fig. 1.

Fig. 1.

Recombinant protein analysis by SDS-PAGE and Western blotting. Lsa20 (A) and rLIC11030 (B) protein expression from NaCl-induced E. coli BL21-SI and IPTG-induced E. coli BL21(DE3) Star pLysS cultures, respectively. Lanes: 1 and 6, molecular mass protein marker; 2, noninduced total bacterial extract; 3, total bacterial cell lysates after induction; 4 and 5, soluble and insoluble (pellet) fractions of the induced culture, respectively; 7, purified recombinant proteins in Coomassie blue-stained gels; 8, Western blotting of the recombinant proteins probed with anti-His tag antibodies.