Fig. 7.
Inhibition of L. interrogans attachment to laminin or to plasminogen by Lsa20. Laminin or plasminogen (1 μg/well) was adsorbed to microtiter plates followed by incubation with increasing concentrations of Lsa20 (0 to 7.50 μg) for 90 min at 37°C. Live leptospires (100 μl/well of 4 × 107 L. interrogans serovar Copenhageni strain M20 leptospires) were added for 90 min at 37°C. The unbound leptospires were washed, and the quantification of bound leptospires was performed indirectly by anti-LipL32 antibodies produced in mice (1: 4,000 dilution) followed by horseradish peroxidase-conjugated anti-mouse IgG antibodies. Each point represents the mean absorbance value at 492 nm ± standard deviation of three replicates. Data are representative of two independent experiments. *, P < 0.009; •, P < 0.005.