Fig. 2.

OMVs package outer membrane and periplasmic proteins and contain surface and luminal DNAs. (A) The OMV protein profile was compared to NTHI strain 86-028NP cytoplasmic (CP), periplasmic (PP), and outer membrane (OMP) proteins isolated from whole cells. Protein preparations were separated by SDS-PAGE and visualized by silver staining. (B) Surface-associated and luminal DNAs were quantified for OMVs, OMVs treated with DNase, and the OMV pellet and supernatant following incubation with GES lysis reagent. Error bars represent the standard errors of the means for three independent assays repeated in triplicate (n = 3).