Table 1.

NTHI 86-028NP OMV proteins identified by LC-MS/MS

Identified protein (over 95% confidence)	Gene no.	GenBank accession no.	Molecular mass (kDa)	No. of protein hitsa
Outer membrane protein P2 precursor	NTHI0225	68056946	40	135
Outer membrane protein P5	NTHI1332	68057911	38	46
Probable amino acid ABC transporter binding protein	NTHI1243	68057836	28	34
HMW1B (OMP-85-like protein required for secretion of HMW1A and HMW2A)	NTHI1984	68058015	61	34
HMW2B (OMP-85-like protein required for HMW1A and HMW2A secretion)	NTHI1449	68058015	61	34
IgA-specific serine endopeptidase	NTHI1164	68057774	197	32
Outer membrane protein P1 precursor	NTHI0522	68057203	50	30
Probable d-methionine-binding lipoprotein MetQ	NTHI0877	68057518	30	27
NAD nucleotidase	NTHI0303	68057010	66	26
Heme-binding protein A	NTHI1021	68057646	61	26
Spermidine/putrescine-binding periplasmic protein 1 precursor	NTHI1823	68058340	43	26
Probable periplasmic serine protease do/HhoA-like precursor	NTHI1905	68058410	49	25
Heme utilization protein	NTHI1390	68057962	103	25
l-Lactate dehydrogenase	NTHI2049	68058536	42	25
Hemoglobin-haptoglobin binding protein B	NTHI0782	68057439	114	24
Putative periplasmic chelated iron binding protein	NTHI0481	68057164	32	23
2′,3′-Cyclic-nucleotide 2′-phosphodiesterase	NTHI0741	68057402	73	22
Periplasmic oligopeptide-binding protein	NTHI1292	68057874	61	22
Phosphate-binding periplasmic protein precursor PstS	NTHI1774	68058299	37	18
Elongation factor Tu	NTHI0712	68057378	43	17
Putative lipoprotein	NTHI1957	68058456	63	16
Protective surface antigen D15	NTHI1084	68057702	88	16
Iron utilization periplasmic protein hFbpA	NTHI0177	68056907	36	15
Glycerol-3-phosphate transporter	NTHI0809	68057464	53	15
TRAP-type C4-dicarboxylate transport system, periplasmic component	NTHI0232	68056953	36	14
HMW1A (high-molecular-weight adhesin 1)	NTHI1983	68058480	154	14
d-Galactose-binding periplasmic protein precursor	NTHI0987	68057616	38	10
Conserved hypothetical protein	NTHI1208	68057809	41	10
TolB	NTHI0502	68057184	45	10
Conserved hypothetical lipoprotein	NTHI0266	68056981	29	10
Glycerophosphoryl diester phosphodiesterase precursor	NTHI0811	68057465	42	10

^aPurified OMVs isolated from 86-028NP were analyzed using LC-MS/MS and were matched with the NCBI H. influenzae 86-028NP database. Listed proteins include those with 10 or more protein hits, all of which have over 95% confidence. A full list of identified proteins (142) can be found in Table S1 in the supplemental material.