Table 1.
Concentrations of IL-6 and chemokines in the sera of mice infected with Y. pestis KIM5
| Cytokine or chemokine | Y. pestis dose | Mean concn (pg/ml) ± SD in seruma |
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|---|---|---|---|---|---|---|---|
| Young B10.T(6R) mice |
Middle-aged B10.T(6R) mice |
C57BL/6 mice |
|||||
| Uninfected | Infected | Uninfected | Infectedb | Uninfected | Infectedb | ||
| IL-6 | 102 | ND | 105 ± 6 | ND | 517 ± 16*** | ND | 358 ± 8*** |
| 103 | ND | 205 ± 8 | ND | 1,207 ± 80*** | ND | 355 ± 17* | |
| CCL5 | 102 | 105 ± 2 | 138 ± 5 | 92 ± 1 | 337 ± 7*** | 81 ± 2 | 226 ± 8*** |
| 103 | 119 ± 9 | 168 ± 6 | 62 ± 2 | 1,184 ± 38*** | 100 ± 1 | 999 ± 40*** | |
| KC | 102 | 65 ± 2 | 519 ± 11 | 50 ± 4 | 1,076 ± 56* | 70 ± 8 | 3,538 ± 338*** |
| 103 | 71 ± 0.5 | 998 ± 61 | 76 ± 3 | 3,309 ± 70*** | 71 ± 0.6 | 2,468 ± 167*** | |
| MCP-1 | 102 | 307 ± 40 | 2,435 ± 39 | 288 ± 12 | 8,027 ± 108*** | 323 ± 44 | 4,919 ± 258*** |
| 103 | 321 ± 45 | 2,231 ± 27 | 379 ± 2 | 6,023 ± 50*** | 310 ± 22 | 7,069 ± 290*** | |
a
Determined 3 days postinfection for the pooled sera of mice (n=6) infected with 1 × 102 CFU or 1 × 103 CFU of Y. pestis KIM5. Serum cytokine or chemokine concentrations were determined using a protein suspension array assay. The SD for each sample in triplicate is given. Results are from individual experiments with two infectious doses. ND, not detected.
b
*, P < 0.05; ***, P < 0.001. The statistical significances of the differences between young and middle-aged B10.T(6R) mice and between young B10.T(6R) mice and B6 mice were determined using one-way analysis of variance (Tukey's multiple-comparison posttest).