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. 2011 Nov;79(11):4523–4532. doi: 10.1128/IAI.05412-11

Fig. 1.

Fig. 1.

Structure and recombinant proteins of GAMA. (A) Schematic of the primary structure of GAMA. The GAMA protein consists of 738 amino acids, with a calculated molecular mass of 85.24 kDa. Indicated are the predicted signal peptide (SP; residues 1 to 24), asparagine-rich region (residues 356 to 485), and C-terminal transmembrane domain (TM; residues 715 to 738). FL GAMA (residues 1 to 738) and three regions of GAMA (Tr1 [residues 25 to 337], Tr3 [residues 500 to 714], and ECTO [residues 25 to 714]) were expressed in recombinant form and used to raise specific antisera. (B) SDS-PAGE of recombinant proteins of GAMA. All the recombinant proteins were synthesized using a wheat germ cell-free protein expression system. The fractions of purified ECTO (79.82 kDa), Tr1 (36.8 kDa), Tr3 (24 kDa), and FL (85.24 kDa) proteins resolved in an SDS-PAGE gel and stained with Coomassie brilliant blue R-250 are shown. M represents a molecular weight marker. E1 and E2 represent the first and the second fractions of purified proteins eluted from affinity purification columns, respectively. Arrows indicate specific bands.