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. 2011 Nov;79(11):4523–4532. doi: 10.1128/IAI.05412-11

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Fig. 3. — Localization of GAMA in asexual blood-stage parasites. (A) GAMA localization using an immunofluorescence assay. Acetone-fixed P. falciparum 3D7 mature schizonts (top panel) and free merozoites (bottom panel) were probed with rabbit anti-FL (green) and mouse anti-PfAMA1 (microneme marker) (red). Parasite nuclei were stained with DAPI (blue). Scale bars represent 2 μm. (B) GAMA localization using immunoelectron microscopy. The two sections of merozoites in schizont-infected erythrocytes were probed with purified rabbit anti-Tr3 antibody and subsequently by secondary antibody conjugated with gold particles. The arrows indicate the micronemal localization of signals from gold particles. Bars represent 200 nm. Arrows mark micronemes. R's mark rhoptries.