Fig. 3.

Kinetics of FnCW-induced release of hBD-2 and hBD-3 by HOECs. (A and B) HOEC monolayers (mixed donors; N = 3) were challenged with FnCW (10 μg/ml) for the indicated time periods, and the levels of hBD-2 and hBD-3 in cell-free supernatants were determined by ELISA. Results are the means ± standard deviations (SD) of three independent experiments. (C and E) Fold changes (calculated from panels A and B) in hBD-2 and -3 release by HOECs (mixed donors; N = 3) after FnCW challenge for the indicated time periods. Results are the means ± SD of three independent experiments. (D and F) Interpersonal variability in hBD-2 and -3 release by HOECs from five different donors after FnCW treatment for 24 h. Results are the means ± SD of three independent ELISA measurements.