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Comparison of FnCW, IEF fraction of FnCW-induced and recombinant FAD-I (rFAD-I)-induced CCL20 and hBD-2 release. HOECs (mixed donors; N = 3) were treated with medium alone, FnCW (10 μg/ml), IEF fraction (10 μg/ml), or rFAD-I (10 μg/ml) for the indicated time periods. The levels of CCL20 (A) and hBD-2 (B) in cell-free supernatants were measured by ELISA. The IEF fraction and rFAD-I were prepared as described by Gupta et al. (29). *, P < 0.05; NS, not significant.
