Fig. 5.

Release of LDH from BMDMs infected with IP2666 or ΔyopJ at different MOIs. As indicated, BMDMs were infected with either parental wild-type strain IP2666 or strain ΔyopJ at an MOI of 10, 20, 40, or 100 as described in the legend to Fig. 2 with 20 min of contact time and 1 h of incubation with 8 μg/ml of Gm followed by 23 h of incubation with 4.5 μg/ml of Gm. Alternatively, IP2666 in 0.5 ml of tissue culture medium was added to one well of BMDMs in 0.5 ml of medium containing 16 μg/ml of Gm at the indicated MOI (IP2666Gm) and incubated for the remaining time. The amount of LDH released was determined at the end of the incubation. Results shown are from one representative experiment of three. The level of LDH released from cells left uninfected is indicated with a dotted line. P values were determined by two-way analysis of variance and Bonferroni posttests where indicated. *, P < 0.05 for comparison of the values from IP2666 and either IP2666Gm or ΔyopJ of the same MOI; ^, P < 0.05 for comparison of the values between IP2666Gm and ΔyopJ.