Fig. 7.
Detection of translocated Yop-bla fusion protein expressed from intracellular Y. pseudotuberculosis. BMDMs were infected with ΔyopJ or ΔyopB strains carrying pYopH-bla (YopH-bla) or pYopJC172A-bla (YopJ-bla). After 1 h of treatment with 8 μg/ml Gm, IPTG was added and included during the last 3 h of infection. BMDMs were then incubated with CCF2-AM for 1 h. Green and blue images were captured by fluorescence microscopy. Arrowheads indicate some blue cells that contain translocated Yop-bla fusion proteins.