Table 2.
Bactericidal titers of NhhA-specific polyclonal antibodies
| Strain | Titera |
Remarks | |
|---|---|---|---|
| Anti-NhhA | Anti-SEAM 12b | ||
| Natural isolate | |||
| MC58 | 256 | 65,536 | Trimer |
| M4407 | 4,096 | 65,536 | Trimer |
| 2996 | 512 | 32,768 | Trimer |
| 8047 | 256 | 8,192 | Trimer |
| NZ05/33-derived recombinant strains | |||
| NZ05/33 | <16 | 16,384 | Monomer |
| NZ05/33ΔNhhA | <16 | 16,384 | Deletion mutant |
| NZ05/33_D549 | <16 | 16,384 | Monomer |
| NZ05/33_D549G | <16 | 8,192 | Trimer |
| M1390-derived recombinant strains | |||
| M1390 | 16 | 65,536 | Monomer |
| M1390ΔNhhA | <16 | 65,536 | Deletion mutant |
| M1390_D549 | 16 | 65,536 | Monomer |
| M1390_D549G | 1,024 | 65,536 | Trimer |
a
Bactericidal titers obtained using rabbit complement. The titers are expressed as the reciprocal of the serum dilution necessary to obtain >50% bacterial killing.
b
The SEAM12 monoclonal antibody against capsule polysaccharide was used as a positive control; a difference of a single dilution was not considered significant.