Fig. 1.

Role of ERdj3 in CT intoxication. (A) CHO cells transfected with plasmid expression vectors encoding nothing (Empty Vector), wild-type ERdj3 (wtERdj3), or dominant negative ERdj3 (dnERdj3) were exposed to the stated concentrations of CT for 2 h. The extent of intoxication was determined from the elevated levels of intracellular cAMP. Results are presented as percentages of the maximal cAMP response for all tested conditions. (B) CHO cells transfected with plasmid expression vectors encoding nothing, wtERdj3, or dnERdj3 were exposed to the stated concentrations of DT for 4 h. The extent of intoxication was determined from the incorporation of radiolabeled methionine into newly synthesized proteins. Results are presented as percentages of the maximal signal obtained from unintoxicated cells. Data in both panels represent the means ± standard errors of the means from 4 independent experiments with triplicate samples for each condition.