Fig. 4.
ERdj3 affinity for CTA1 and CTA11–168. (A and B) ERdj3 was perfused at concentrations of 1,600, 800, 400, 200, and 100 ng/ml over SPR sensor slides coated with CTA1 (A) or CTA11–168 (B). ERdj3 was removed from the perfusion buffer 300 s into the experiment. (C and D) CTA1 (C) or CTA11–168 (D) was perfused at concentrations of 1,600, 800, 400, 200, and 100 ng/ml over an ERdj3-coated SPR sensor slide. Toxin was removed from the perfusion buffer 300 s into the experiment. For all experiments, perfusion was at pH 7.4 and 37°C. Measurements collected from three independent experiments per condition are shown. The orange lines represent best-fit curves derived from the raw data using 1:1 binding models.