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Detection of TRP120 expression in yeast. Expression of GAL4 DNA-BD-TRP120 fusion protein in yeast (arrow) was determined by immunoblotting of yeast cell extracts transformed with pGBKT7 (empty vector) or pGBKT7-TRP120 using the anti-TRP120 antibody or anti-GAL4 DNA-BD antibody. M, molecular mass marker.
