Confirmation of positive interactions in yeast by cotransformation. Y2HGold yeast cells were cotransformed with bait plasmid pGBKT7-TRP120 and prey plasmid pGADT7-IGL, -COX2, -GGA1, -PCGF5, -ACTG1, or -UNC13D. As a control, pGBKT7 (empty vector) was used to cotransform with each prey plasmid. The positive interactions were confirmed by selection on QDO/X/A plates.