Table 2.
Histological lesion grading in organs of animals inoculated with virulent L. interrogans serovar Icterohaemorrhagiae
| Organ and pathology | Lesion score at indicated times postinfection (days)a |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Hamsters |
Mice |
|||||||||||
| 0.5 | 1 | 2 | 3 | 4 | 4.5 | 0.5 | 1 | 2 | 3 | 5 | 15 | |
| Liver | ||||||||||||
| Edema and congestion | − | 3 | 5 | 6 | 6 | 9 | − | − | − | − | − | − |
| Portal inflammation | − | 1 | 3 | 3 | 3 | 6 | − | 1 | − | 2 | 3 | 2 |
| Hepatocyte necrosis | − | 2 | 1 | 4 | 3 | 7 | − | − | − | 2 | 3 | 2 |
| Regeneration | − | 1 | − | 4 | 3 | 9 | − | − | 1 | 3 | 3 | − |
| Kupffer cell hyperplasia | − | − | 1 | 4 | 1 | 8 | − | − | − | − | − | − |
| Kidneys | ||||||||||||
| Interstitial nephritis | − | − | − | − | − | − | − | − | − | 2 | 1 | − |
| Congestion | − | − | − | 3 | 5 | 9 | − | − | − | 1 | − | − |
| Hemorrhage | − | − | − | − | − | 9 | − | − | − | − | − | − |
| Lungs | ||||||||||||
| Edema | − | 3 | 4 | 6 | 6 | 6 | − | 1 | 1 | 1 | 4 | − |
| Alveolar hemorrhage | − | 2 | 3 | 5 | 9 | 8 | − | 1 | − | − | 3 | − |
| Inflammation of alveolar septa | − | 2 | 3 | 3 | 5 | 6 | − | − | 1 | 1 | 3 | − |
a
Hamsters and mice were intraperitoneally infected with virulent L. interrogans serovar Icterohaemorrhagiae strain Verdun. Scores were obtained from addition of individual scores (n = 3) after observation of HES-stained sections of each organ. −, no lesion; 1 or 2 (white), slight lesions; 3 or 4 (gray shading), moderate lesions; ≥5 (boldface), severe lesions.