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. 2011 Nov;79(11):4688–4695. doi: 10.1128/IAI.05289-11

Fig. 3.

Fig. 3.

IFN-γ production by spleen cells induced by B. abortus is partially dependent on IRAK-4. IRAK-4−/− and IRAK-4+/− mice were infected and spleens were harvested for splenocyte culture. Spleen cells were stimulated with B. abortus S2308 (MOI, 100:1), HKBa (MOI, 100:1), TLR2, or TLR9 agonists (Pam3CSK4 or CpG, 1 μg/ml of each). After 72 h, supernatants were harvested for measuring IFN-γ levels by ELISA. IFN-γ was measured at 1 (A), 3 (B), and 6 (C) weeks postinfection. These results are representative of three independent experiments. A significant difference in relation to nonstimulated cells is denoted by #, and one in relation to IRAK-4+/− mice is denoted by an asterisk (P < 0.05). ConA, concanavalin A.