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. 2011 Nov;79(11):4688–4695. doi: 10.1128/IAI.05289-11

Fig. 4.

Fig. 4.

IL-12 and TNF-α production induced by B. abortus in macrophages is IRAK-4 dependent. Bone marrow from IRAK-4−/− and IRAK-4+/− mouse cells was differentiated in macrophages and stimulated with B. abortus S2308 (MOI, 100:1), HKBa (MOI, 100:1), Pam3CSK4 (1 μg/ml), CpG (1 μg/ml), Brucella L-Omp19 lipoprotein (5 μg/ml), Brucella DNA (DNA B.a.; 1 μg/ml), or E. coli LPS (1 μg/ml). Supernatants were harvested for measuring IL-12 (A) and TNF-α (B) after 24 h by ELISA. A significant difference in relation to nonstimulated cells is denoted by #, and a significant difference in relation to IRAK-4+/− mice is denoted by an asterisk (P < 0.05).