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. 2012 Jan 13;90(1):142–151. doi: 10.1016/j.ajhg.2011.11.027

Figure 5.

Figure 5

C12orf62-FLAG Coimmunoprecipates with COX I, II, and IV and with LRPPRC, EFTu, and SLIRP

(A) Mitochondria from either fibroblasts expressing C12orf62-FLAG or control fibroblasts were extracted and incubated with anti-FLAG agarose beads. Immunoblotting with antibodies against FLAG, COX I, COX II, and COX IV was performed on each fraction.

(B) Mitochondria from control fibroblasts expressing C12orf62-FLAG were extracted and incubated with magnetic beads that either were uncoated or were coated with COX I or COX II antibodies. Immunoblotting with antibodies against FLAG, COX I, and COX II was performed on the input, unbound, and eluate fractions.

(C) The immunoprecipitation was performed with FLAG beads as described in (A), and immunoblotting with antibodies against FLAG, LRPPRC, EFTu, and SLIRP was performed on each fraction.