Figure 6.

CBS immunoreactivity and densitometric analysis in kidney slices: CBS immunoreactivity (Brown staining with 3,3- diaminobenzidine) was observed exclusively in intracellular compartment of proximal tubular (PT) cells of paraffin embedded kidney (5 µm) sections of control rats. Similar distribution, though with marked increased immunointensity, was observed in kidney sections from rats treated with combination (PAG + AOA) therapy (panel d). It should be noted that CBS immunoreactivity was mainly observed at the brush border but not intracellularly in the PT cells in rats treated either PAG (panel b) or AOA (panel c) alone. Glomeruli, other tubular segments and renal vasculature were negative. Mean data from the densitometric analysis of the CBS intensity in these kidney sections are illustrated in e. Values are mean ± s.e. *P < 0.05 vs. PAG treated group only. Magnification: 200X. AOA, aminooxyacetic acid; CBS, cystathionine β-synthase; CD, collecting duct; Glom, glomerulus; PAG, -propargylglycine.