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LH response to a bolus injection of GnRH at 4 years of age (A) and 5 years of age (B). GnRH (250 ng/kg, i.v.) was infused at time 0. Testosterone-treated animals showed a significantly greater response measured as area under the curve at 4 years of age (P = 0.042) but not at 5 years of age (P = 0.57). Data are expressed as mean ± SEM.
