Table 1.

Primer sequences

Name of the Primer	Sequence	Length (bases)
E183G-PDH45F	5′-CAA GTT ACT AGT TCT GGA TGG ATC TGA TGA AAT GTT GAG CAG-3′	42
E183G-PDH45R	5′-CTG CTC AAC ATT TCA TCA GAT CCA TCC AGA ACT AGT AAC TTG-3′	42
R363Q-PDH45F	5′-GTA CAT TCA TCG GAT TGG TCA ATC TGG ACG TTT TGG ACG AAA G-3′	43
R363Q-PDH45R	5′-CTT TCG TCC AAA ACG TCC AGA TTG ACC AAT CCG ATG AAT GTA C-3′	43
PDH45-F (NdeI)	5′-AGA GGC ATA TGG CGA CAA CTT CTG TGG-3′	27
PDH45-R (XhoI, EcoRI)	5′-GAG GAA TTC CTC GAG TTA TAT AAG ATC ACC AAT ATT C-3′	37