Fig. 1.

Δψm in PINK1 KO skeletal myocytes, fibroblasts and neurons. (A) Δψm was measured using TMRM in primary neuronal cultures and skeletal muscle and fibroblast cultures. Δψm was reduced in PINK1 KO neurons and fibroblasts, but increased in PINK1 KO myocytes compared with the WT. A summary of mean values normalised to WT (±s.e.m.) is shown: myocytes, 198.7±40.5%; neurons, 63.7±4.2%; fibroblasts, 63.4±7.3%, *P<0.05. (B) Representative traces showing dynamic response of Δψm in response to oligomycin, rotenone and carbonyl cyanide-p-(trifluoromethoxy) phenylhydrazone (FCCP). In WT myocytes, oligomycin did not affect Δψm; rotenone induced partial depolarization; FCCP induced complete depolarization. In PINK1 KO myocytes, oligomycin induced mitochondrial depolarization. (C) Application of 5 mM pyruvate or 5 mM methyl succinate to PINK1 KO myocytes increased basal Δψm and prevented oligomycin-induced mitochondrial depolarization in PINK KO cells. Error bars represent mean + s.e.m.