Fig. 4.
Expression of endogenous EMC protein is increased in rbf1 mutant photoreceptors. Mosaic clones of an rbf1-null allele, rbf1Δ14, were generated in third-instar larval eye discs using eyFLP. The rbf1Δ14 mutant clones are marked by the lack of GFP signal. (A) Anti-EMC antibody (red) was used to compare the expression pattern of EMC in the wild-type and rbf1 mutant clones. The position of the MF is marked by white arrows. The yellow arrow indicates a small rbf1Δ14 clone where EMC expression was increased. (B) Posterior region of an eye disc containing rbf1Δ14 mutant clones. The disc was co-stained with anti-EMC (red) and anti-ELAV (blue) antibodies. Note the overlap between EMC and ELAV signals in the rbf1Δ14 mutant clones, indicating that EMC expression was deregulated in the rbf1Δ14 mutant photoreceptors.