Fig. 1.
Expression of JIL-1 constructs transgenically in a wild-type background. (A) Diagrams of the JIL-1 CFP-tagged constructs analyzed. The region in the CTD where JIL-1Su(var)3-1 alleles resulting in C-terminally truncated proteins have been mapped (Ebert et al., 2004) is indicated by a bracket. (B) Immunoblot labeled with JIL-1 antibody of protein extracts from wild-type (WT) and flies expressing the FL, the CTD and the ΔCTD constructs, respectively. Labeling with antibody against tubulin was used as a loading control. The relative migration of molecular size markers in kDa is indicated to the left of the immunoblot. (C) Immunoblot, labeled with antibody against phosphorylated H3S10 (H3S10ph) of protein extracts from salivary glands from WT third-instar larvae, from larvae expressing the FL, the CTD and the ΔCTD, respectively, and from JIL-1 null larvae (Z2). Labeling with antibody against histone H3 was used as a loading control.