Fig. 3.

The effect on H3K9me2 localization in polytene chromosomes expressing the CTD. The polytene squash preparations were labeled with antibody against H3K9me2 (in red) and with Hoechst (DNA, in blue or grey). The X chromosome is indicated by an X. Preparations from wild-type (control) and male and female larvae expressing the CTD are shown. In wild-type preparations, H3K9me2 labeling was mainly localized to and abundant at the chromocenter – however, when the CTD was expressed, the H3K9me2 labeling spread to the autosomes and particularly to the X chromosome in both males and females.