Fig. 9.

The effect on H3K9me2 localization in polytene chromosomes from JIL-1 null larvae expressing a ‘kinase dead’ JIL-1 construct. The polytene squash preparation is from a male JIL-1 null (JIL-1^z2/JIL-1^z2) third-instar larvae triple labeled with Hoechst (DNA, in blue or grey), H3K9me2 antibody (in red) and JIL-1 antibody (in green). Note that although expression of the ‘kinase dead’ construct is near wild-type levels, and is localized on the chromosome arms and upregulated on the male X chromosome (X), the chromosome morphology as well as the spreading and upregulation of histone H3K9 dimethylation on the X chromosome are indistinguishable from those observed in JIL-1 null third-instar larvae (Fig. 7).