Fig. 2.

The BubR1 N-terminus is essential for SAC. (A) Immunoblots of HeLa cells transfected with an shRNA plasmid targeting BubR1 estimating RNAi efficiency. Bub3 is used as a loading control. (B) Immunoblots showing expression of BubR1 mutants upon tetracycline addition in cells depleted of the endogenous protein. (C) A cumulative plot showing the amount of time nocodazole-treated cells arrest in mitosis following BubR1 complementation. At least 40 cells were analysed in each case. (D) Box and whisker plots showing the time cells take from nuclear envelope break down (NEBD) to anaphase onset. At least 30 cells were analysed for each condition.