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. 2011 Sep 14;40(2):541–552. doi: 10.1093/nar/gkr701

Figure 1.

Figure 1.

Initiation complex formation on uncapped XIAP IRES RNA. (A) Schematic diagram of the DNA construct of the streptomycin aptamer-tagged XIAP IRES RNA used for analysis of initiation complexes by toeprinting and affinity purification. (B) XIAP IRES initiation complexes were formed on the in vitro transcribed RNA with or without poly-A tail in RRL pre-treated with GMP-PNP or GTP and/or ATP. Subsequently, initiation complexes were analyzed by toeprinting. (C) A magnified portion of the toeprinting gel showing toeprints from +17 to +19 downstream of the AUG with the distribution of fluorescence intensities indicated. XIAP IRES initiation complexes 48S, 80S or 48S + 80S were determined as described by Shirokikh et al. (27).