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. 2011 Sep 14;40(2):541–552. doi: 10.1093/nar/gkr701

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Isolation of XIAP IRES initiation complexes formed in poly I:C-treated RRL. RRL was supplemented with purified 40S and 60S subunits from HeLa cells and subsequently treated with poly I:C, ATP and cycloheximide. Initiation complexes were formed on strepto-tagged XIAP IRES RNA and affinity purified using streptomycin affinity chromatography as described by Locker et al. (20). (A) A typical profile of RNA/initiation complex elution from the dihydrostreptomycin column. (B) Agarose gel electrophoresis analysis of the eluate from the streptomycin column. (C) Without further purification 5μl of eluate was analyzed by toeprinting assay for XIAP IRES initiation complexes.