. 2011 Sep 28;40(2):638–649. doi: 10.1093/nar/gkr705

Table 1.

Sequence of oligonucleotides

Oligonucleotide	Sequence (5′–3′)^a
S11A	CGCCAGAGAGCGCGTGTC
S14A	CGCGTGTCGCCCAAATGA
S11/14A	CGCCAGAGAGCGCGTGTCGCCCAAATGA
S11D	CGCCAGAGAGACCGTGTCTCC
BS19	TGCCTGCAGTAATACGACTCACTATAGAAAACTCACCGCAACGA
BS43	GTCGACTCTAGAT₍₁₅₎
pET21-GFP-F	GGAATTCCATATGGTGAGCA AGGGCGAGGA
pET21-GFP-R	CCGCTCGAGCTTGTAC AGCTCGTCCA TGC
T7P	AACTGCAGTAATACGACTCACTATA
T7T	CGGGATCCT₍₁₅₎CAAAAAACCC CTCAAGACCC

All primers were designed corresponding to the sequence of pBSF4 (52).

^aBoldface letters indicate the amino substitution.