Table 1.
N | Name | L | sequence |
---|---|---|---|
1 | d120_c | 120 | AGCGAGTTGATGTTAGACTGTGTACTTTTTGCGAGTTGATGTTAGACTGTGTACTTTTTA AGCGAGTTGATGTTAGACTGTGTACTTTTTGCGAGTTGATGTTAGACTGTGTACTTTTTA |
2 | d60_c | 60 | AGCGAGTTGATGTTAGACTGTGTACTTTTTGCGAGTTGATGTTAGACTGTGTACTTTTTA |
3 | d29 | 29 | AAAAAGTACACAGTCTAACATCAACTCGC |
4 | d29_c | 29 | GCGAGTTGATGTTAGACTGTGTACTTTTT |
5 | d29_cmm | 29 | GCGAGTTGACGTCAGACCGTGCACTTTTT |
6 | d25_c | 25 | GAGTTGATGTTAGACTGTGTACTTT |
7 | d20_c | 20 | GTTGATGTTAGACTGTGTAC |
8 | d15_c | 15 | TTGATGTTAGACTGT |
9 | d10_c | 10 | TTGATGTTAG |
10 | d5_c | 5 | TTGAT |
11 | d53_pT-b | 53 | GAGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGAGC |
12 | d58_pT-b | 58 | GAGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGAGC |
13 | d60_orf1 | 60 | GATCATGCAGGACAGTCGGATCGCAACCTGATTTACTGTGTCATATAGTACGTGATTCAG |
14 | r60_U | 60 | UUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUU |
15 | r30_3′utr-a | 30 | ACCUGCACAAUGUGCACAUGAAAAAAAAAA |
16 | r26_3′utr | 26 | ACCUGCACAAUGUGCACAUGAAAAAA |
17 | r22_3′utr | 22 | ACCUGCACAAUGUGCACAUGAA |
Each oligonucleotide has a prefix (d or r, for DNA or RNA) followed by its length and, in some cases, additional identifying information. Oligonucleotides 1–10 are based on the d29 (or its complement d29_c) oligonucleotide, which are identical to the ones called 29linear and c29linear respectively by Martin and Bushman (45); likewise, d29_cmm is identical to the cmm29linear (45), which when paired with d29 results in four mismatched positions (underlined). Oligonucleotide 2, d60_c, is a dimer of d29_c with an additional 5′ and 3′ A. d120_c (oligonucleotide 1) is a dimer of d60_c. Oligonucleotides 6–10 are each subsets of the d29_c sequence. Oligonucleotides 11–14 are arbitrary DNA sequences, and gel shift assays like those shown in Figure 6 showed little difference between these and d60_c in their ability to form shifted products with ORF1p (data not shown). The sequence of oligonucleotides 15–17 corresponds to positions 5980–6000 of the 3′ UTR of L1.3 (38) followed respectively by 10, 6 and 2 A's.