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. 2011 Sep 21;40(2):813–827. doi: 10.1093/nar/gkr728

Table 1.

Oligonucleotide sequences

N Name L sequence
    1 d120_c 120 AGCGAGTTGATGTTAGACTGTGTACTTTTTGCGAGTTGATGTTAGACTGTGTACTTTTTA AGCGAGTTGATGTTAGACTGTGTACTTTTTGCGAGTTGATGTTAGACTGTGTACTTTTTA
    2 d60_c 60 AGCGAGTTGATGTTAGACTGTGTACTTTTTGCGAGTTGATGTTAGACTGTGTACTTTTTA
    3 d29 29 AAAAAGTACACAGTCTAACATCAACTCGC
    4 d29_c 29 GCGAGTTGATGTTAGACTGTGTACTTTTT
    5 d29_cmm 29 GCGAGTTGACGTCAGACCGTGCACTTTTT
    6 d25_c 25 GAGTTGATGTTAGACTGTGTACTTT
    7 d20_c 20 GTTGATGTTAGACTGTGTAC
    8 d15_c 15 TTGATGTTAGACTGT
    9 d10_c 10 TTGATGTTAG
10 d5_c 5 TTGAT
11 d53_pT-b 53 GAGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGAGC
12 d58_pT-b 58 GAGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGAGC
13 d60_orf1 60 GATCATGCAGGACAGTCGGATCGCAACCTGATTTACTGTGTCATATAGTACGTGATTCAG
14 r60_U 60 UUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUU
15 r30_3′utr-a 30 ACCUGCACAAUGUGCACAUGAAAAAAAAAA
16 r26_3′utr 26 ACCUGCACAAUGUGCACAUGAAAAAA
17 r22_3′utr 22 ACCUGCACAAUGUGCACAUGAA

Each oligonucleotide has a prefix (d or r, for DNA or RNA) followed by its length and, in some cases, additional identifying information. Oligonucleotides 1–10 are based on the d29 (or its complement d29_c) oligonucleotide, which are identical to the ones called 29linear and c29linear respectively by Martin and Bushman (45); likewise, d29_cmm is identical to the cmm29linear (45), which when paired with d29 results in four mismatched positions (underlined). Oligonucleotide 2, d60_c, is a dimer of d29_c with an additional 5′ and 3′ A. d120_c (oligonucleotide 1) is a dimer of d60_c. Oligonucleotides 6–10 are each subsets of the d29_c sequence. Oligonucleotides 11–14 are arbitrary DNA sequences, and gel shift assays like those shown in Figure 6 showed little difference between these and d60_c in their ability to form shifted products with ORF1p (data not shown). The sequence of oligonucleotides 15–17 corresponds to positions 5980–6000 of the 3′ UTR of L1.3 (38) followed respectively by 10, 6 and 2 A's.