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. 2011 Sep 27;40(2):726–738. doi: 10.1093/nar/gkr748

Figure 4.

Figure 4.

BRCA1 and RAD51 proteins are localized at DNA ssDNA or DSBs region in response to stalled or collapsed DNA replication forks. (A and B). Kinetics of RAD51 (A) or BRCA1 (B) focus formation. The percentage of cells with more than 10 nuclear foci was calculated. In each experiment, 200 nuclei were counted per data point. Error bars indicate standard errors from three independent experiments (*P < 0.01). (C) BRCA1 and RAD51 colocalizes at the ssDNA region in response to replication fork stalling (left panel). The protocol for ssDNA detection has been described in a previous publication (12,51). Cells treated with 6-h HU were co-stained by anti-BrdU antibody and antibody against RAD51 or BRCA1. Representative foci images in response to 6-h HU treatment were shown. BRCA1 and RAD51 protein colocalizes at DSBs region in response to replication fork collapse (right panel). The cells co-stained with anti-γ-H2AX antibodies and antibodies against RAD51 or BRCA1. Representative foci images in response to 18-h HU treatment were shown.