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. 2011 Sep 24;40(2):625–637. doi: 10.1093/nar/gkr754

Figure 5.

Figure 5.

(A) Mutation of NFR1 inhibits CFTR promoter activity more profoundly than several CF disease-associated mutations. 16HBE14o- cells were transfected with pGL3B luciferase reporter constructs containing the 2-kb CFTR greater promoter region (pGL3:2kbProm) and a β-galactosidase transfection control plasmid. Promoter mutants: 33G>A, −94G>T, −102T>A, NFR1mut, −329C>T and NFR4mut are shown relative to the CFTR basal promoter-alone vector. (B) Mutation of NFR1 in the ANGPTL3 promoter decreases promoter activity in Caco2 cells. Error bars represent standard errors of the mean [n = 6 or 9 (CFTR and ANGPTL3)]. P-values generated by comparison to the wild-type promoter-only vector by using unpaired t-tests with Welch's correction. Experiments were done a minimum of three times and with more than one plasmid preparation of each construct and results were consistent between them.