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Mediator does not affect heterochromatin at the HMR locus. (A) Expression of endogenous HMRa1 gene was examined. (B) Mediator does not affect expression of HMRa1 in sir2Δ strains. (C) Schematic diagram of HMR region. Primers for ChIP assay were indicated. (D) Sir2 binding at HMR region in Mediator mutants.
