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. 2011 Sep 19;40(2):692–700. doi: 10.1093/nar/gkr761

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The presence of histone H1 strongly inhibits the efficiency of OGG1. ³²P-end labelled control or H1 containing dinucleosomes with either 75 bp (A) or 20 bp (B) linker DNA with a single 8-oxoG inserted within the linker DNA were incubated with 0.2 U of OGG1 for the times indicated. The cleaved DNA was then isolated and separated on 8% denaturing PAGE. The positions of the full length (FL) and the cleaved (cut) DNA are indicated. The lower panels show the quantified data presented as percentage of cut fractions. Data were least square fitted as described in the experimental section and Figure 1F. The experimental points were fit to the same R_m = 83% that was obtained for the –H1 data and then fixed for the +H1 data point fitting. The mean values from two independent experiments for of T = t_c⁻^H1/t_c⁺^H1 were: 8.1 ± 12.4% and 10.3 ± 13.2% for the 75- and 20-bp linker DNA, respectively.