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. 2011 Sep 19;40(2):692–700. doi: 10.1093/nar/gkr761

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Removal of histone H1 and RSC remodelling of the dinucleosome are necessary for efficient repair of 8-oxoG within the nucleosomal DNA. The 20-bp linker dinucleosomes, containing a single 8-oxoG in close vicinity to the nucleosome dyad, (see schematics at upper panel) with or without histone H1 were treated with either 2.5 U of RSC or 18× excess of NAP-1 or with both as indicated. At the same time the samples were treated with 1 U of OGG1 for 90 min and the cleaved DNA was run on 8% denaturing gel (middle panel). The position of the full length (FL) and cleaved (cut) DNA are indicated; 1/2, half of the amount of RSC; *indicates the position of the ³²P radioactive label. Quantified data are shown on the lower panel as percentage of cut fractions. The relative SD is ±10–12% for lines 1–4 and ±3.5–5% for lines 5–10.