Figure 6.

OGG1 cleavage of a mixture of two dinucleosome templates containing a single 8-oxoG either in the linker or within the core particle DNA. The 20-bp linker H1 containing dinucleosomal templates were mixed in equimolar amounts and incubated either with NAP-1 (18× excess) alone or with both RSC (2.5 U) and NAP-1 as indicated. The samples were simultaneously treated with 1 U of OGG1 and the cleaved DNA was run on 8% denaturing gel (upper panel). The arrows in the schematics (left) show the site of cleavage and indicate the mobility of the cleaved dinucleosomal template either in the linker (lower mobility) or in the core particle DNA (higher mobility). After exposure of the dried gel, the percentage of cut fraction within both the linker and the core particle DNA was measured (lower panel). The relative SD is ±11% for the two lowest values in lines 1 and 2 and ±3.5–4% for the remaining values.